Blurry green image

Request Help
#1

Really not sure what the problem is, but I’m so close to getting it work.

The connectivity problems have been solved, thanks to suggestions from other users here, but now I’m having difficulty getting a clear image of the slide material.

The image comes up as a bright green, and I’m not sure what the cause of it is. I’ve tried disconnecting and reconnecting the Pi2 camera, on both ends, and that didn’t seem to make any impact.

Even after unscrewing the 40x microscope objective, the image is still green.

But the camera seems to be detecting the material on the slide, displaying shadowy globs here and there within the green.

Is there an optimal material to put on the slide for testing? Right now I’m using saliva, but maybe that isn’t the best choice.

Also, the piece that the pi camera is mounted to was printed with white PLA, which seems to let in some light. Might that factor in to the problem? For the time being, I’ve wrapped it in black electrical tape, because I was also experiencing difficulty in printing out another piece in black PLA. The base of the STL seems to be easily ripped apart by how quickly the nozzle on my printer moves, so I might just spray-paint the white piece black, if needed.

#2

image
image832×624 34.7 KB

#3

This issue ,I have also faced and keep facing.I keep autocalibrating using camera again and again and sometime by chance gets corrected on its own.The probability increases whenever I calibrate more than once…though I don’t know the logic.

#4

Yeah I’m able to zoom in an out, but the screen is still almost completely green, seemingly no matter what sequence of focus/adjust/calibrate I try.

When you say it occasionally corrects itself, do you mean the color of the image is corrected? Or is color not your problem?

#5

I am not sure about the green. Having the optics holder in white will let in stray light. Do it black if you can.
I think a better sample slide will help to separate any different issues. Saliva is not high contrast and it is hard to know whether you can see anything. Prepared stained slides are really good and pretty easy to get hold of. Unstained slices of plant are pretty transparent and again it can be hard to see. Not great if you are not already confident in your microscope. A few hairs gives high contrast black lines to get an idea of the focus.

1 Like
#6

I have a H&E stained section of brain. It should be generally be pinkish.However I have been struggling to calibrate the colors.Its been almost two days,I have been repeatedly trying to get it back to normal, however I have failed.I have attached the screenshot of the image.

Screenshot_20201104-195820
Screenshot_20201104-195820864×864 165 KB

#7

That image shows that the setup and focusing is giving a sharp image. The green cast is very strong. I am clutching at straws here, but I would try removing any colour calibration, or reinstalling the software from scratch. Somewhere a colour correction has gone mad.
I assume you can get a sensible colour picture with the Norma Pi camera capture software?

#8

Thank you.I ll give it a try.

#9

Installed the raspian openflexure afresh…got back my image…working fine…Thanks @WilliamW

1 Like
#10

I’ll give that a shot as well.

#11

The green colour cast during calibration seems to pop up for a lot of people, and I’m currently trying to pin down why; unfortunately, all my test setups don’t show it! I’d be very interested in seeing the output from running ofm log on your microscope if you have recently done a calibration that went green.