I have some issues with color in my microscope images. I expected this to come from a calibration issue in the software but I continue to have the same problem even after this beta fix (thanks for it !).
To explain : I’m taking pictures of algae with the high resolution optics mounted with a 40x objective. I also even glued an iris on the illumination to get finer light adjustment.
I tried multiple illumination settings and calibrations but nothing do, I get only faded green color in my images (when I get crispy green color on a commercial microscope).
On these pictures exposure is approximately around 600 in the Openflexure software, that’s the value I usually get the better results with.
I also observed that when take an image with a stock Pi camera objective, I get a green coloured image :
If anyone had some tips or fix to get images with a better color that would be really helpful !
I performed a white calibration with the stock Pi camera optics on a white piece of paper and managed to get some color on the pic. I did not touch the white calibration and screwed the camera on the high res optics, still no color… (see pic below for the white calibration and cells image)