Hello all. I am planning to build the microscope with the Delta Stage. I noticed that in the paper (Optica Publishing Group) it is said that Fourier ptychography is possible through the use of extensions. I am wondering if anyone has attempted this, and if successful could share some images?
Thank you!
I don’t know wheter it has been done, but it is something that I do want to try.
I think that the ordinary OpenFlexure Microscope is probably the better platform for it. You should only need xy motion.
You also require the LED grid based illumination, or is this not unique only to the Delta Stage?
You can use the LED grid on either type of body, the illumination mounting geometry is the same. The LED grid was developed on the Delta Stage, and that is where the parts and instructions are.
Ptychography would normally use an aperture and move the sample, I thought.
Thanks for the information! That is good to know. I am still nearly learning about the Openflexure design.
Regular (real space) ptychography does indeed move the sample, Fourier ptychography instead moves the illumination angle.
Hi there!
I’ve previously spoken to someone at Heidelberg who had a go at Fourier ptychography - he used the Cartesian stage with an extra translation stage bolted on for illumination upright-style, but had some trouble with suspending his LED matrix with good alignment. He did get some decent images out; as far as I can tell he didn’t want to post to the forum yet. As far as I’m aware it hasn’t been tried in a Delta stage!
Interesting! I’m interested in how exactly the extra translation stage was used!
Do you happen to know what was his achieved illumination NA and effective NA were? I can imagine that LED matrix alignment would certainly be tricky. I think there are ways to correct for this right? I think it could be possible to infer the true position of each LED in space.