… specifically for cyanobacteria and/or chlorophyll-a?
I’ve nearly completed my first delta stage microscope (also my first open source project period) and I’m really excited because this has been a long process of learning & acquiring both skills and parts. I never went to college and I’ve more or less stumbled into being an (extremely) amateur biologist, so I’ve been carefully collecting material on fluorescence microscopy — though this is pretty challenging because i have no background or frame of reference for a lot of the academic material. This brings me to my dilemma:
How do I choose my excitation and emission filters so that they match the purpose of what I’m using the microscope for: observing local microalgae/cyanobacteria (really anything in the pondwater though)? By digging through the many open source fluorometer projects I was able to atleast narrow down the excitation filter i need for observing chlorophyll-a (#19 red from the roscolux booklet) but now I’m at a loss for the emission filter.
Does anyone have any experience they could quickly share?
i want to contribute something so let me add that when building the reflection module I found using a dremel way easier than the suggested oil glass cutter recommended in the instructions. the beam splitter is such a small piece of glass and the budget-friendly tool i sprung for wasn’t delicate/accurate enough. the dremel was much simpler in case anyone else is having problems with cutting the glass*
Thank you for everything.