Thank you for your great initiative that makes imaging feasible for everyone.
I am going to make a fluorescence version of the microscope and have a couple of questions. Hope someone can help me in this regard.
I am mainly focusing on GFP imaging in plants at the beginning.
- Can you suggest a good place and cat. #s to buy the excitation, emission filter, dichroic beam splitter in United States? I talked with Thorelab technical support, they couldn’t give me a catalog number that specifically fits with the Openflexure modules.
- How I can come up with a way to take images removing the chlorophyll autofluorescence that interfere with GFP in Openflexure setup. Here is the link about the issue Please note that I would like to have three images for my studies (autofluorescence, GFP and Brightfield)
- What is the recommended blue LED for the GFP imaging?
Sorry for asking a lot of questions. Thank you in advance.