About v6 reflection illumination microscope

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1

Hi all,

I’m trying to build a v6 reflection illumination microscope. The ‘reflection illuminator.stl’ file includes three parts, but I can’t work out how to use one of the circular parts with a hole in the centre. The instructions don’t mention the parts.

Any information will be much appreciated. Best regards!

BobEndo

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Hi @BobEndo, a picture woild help to understand which part you are lookimg at. The reflection optics option has never had the attention to the instructions that the transmission has had.

Is there a particular reason that you are building v6 rather than v7 for your microscope?

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Thank you for your reply @WilliamW

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I indicate the parts in question with a red circle in the image.

The reason I am building a v6 microscope is that I am trying to build a magneto-optical Kerr microscope, as described in the paper I refer to. In that paper, they constructed a microscope based on the v6 microscope.

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In the code it is called the field_stop(). I think that it goes here:

image
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But it is not clear which way round it goes, probably the way round in the picture. @r.w.bowman?

However I think that this part is changed in the paper (Development of a magneto-optical Kerr microscope... | F1000Research), and is angle_part.stl.

Overall looking at the paper, you should be able replicate this relatively easily with the microscope v7. The optics module mounting and the illumination mounting should be the same. I am not sure why the authors of the paper made the extra cut-out in the front of the body, it might be that they just needed the optics module to go higher up because of a particular objective. Replicating exactly what they did first is a good idea, but after that moving to v7 would be a great idea.

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thank you for your kind instruction.

According to the paper, the extra cut-out is need to turn angle_part.stl

I think the author may attach a polarizer to angle_part.stl, but I can’t understand how to do that.

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The field stop should be the same distance from the objective as the camera sensor - that is fairly close to the position William shows, but it needs a bit of manual tweaking to get it quite right.

This bit of the design was never optimised as much as I’d like… I’m aware there are others who have developed fluorescence implementations that they prefer - but while they’ve expressed a desire to share it with the community, I am not aware of any files being shared yet (and I’ve not tested it myself).

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