Hello! I have a sample with significant topography that I am scanning using the OFM software. I have been trying to modify the autofocus extension to be better at autofocussing on such a sample but I am having trouble. As I understand, in the standard autofocus initially the focus moves up (or down…

Hi @Ryan the step size and range are set when you call the autofocus method; if you’re using the web interface, the parameters are baked in to the code that calls “coarse/medium/fine”. Currently the only way to customise what happens when you click the button would be to modify the web app, but it’…

If you direct me to the section of code that determines the step size and range in the web app I should be able to play around with the parameters. I think having it baked into the code is easier as I perform numerous scans per day. Thanks!

Sure - I am guessing you want to change the autofocus parameters used in the scan, rather than just when you click the autofocus buttons? If so, the relevant file is openflexure_microscope/api/static/src/components/tabContentComponents/captureComponents/paneCapture.vue line 379, that defines the st…

Richard and Ryan, I have attempted to do the same thing to improve the autofocus when scanning at larger intervals using a 10x lens. I was able to test the optimal range for the autofocus using the web browser interface and found where to modify the paneCapture.vue file. I have read the ReadMe fil…

Hi Nicolas To rebuild the webapp after making some changes, the instructions are here [image] webapp · v3 · OpenFlexure / openflexure-microscope-server · GitLab API server and web interface for the OpenFlexure Microscope. The key points are that you need np…

[image] r.w.bowman: http://microscope.local:5000/api/v2/docs/swagger-ui/ This is so awesome that one can play with the parameters here! On the low res OFM I can typically take steps of 5000 and still get good overlap for stitching, but I wish I could extend the autofocus range from the defaul…

You may need to rebuild it to make it persistent - it’s worth saying that our development effort is now all focused on v3, so I’m less and less familiar with the v2 codebase. Scanning is much, much improved in the newer version - though it’s also more tightly optimised so it doesn’t (yet) have as ma…

I am so eager to try v3! When do you think it will be out? Can one already try it out?

Sure, there’s an alpha release, I think there’s a thread in “announcements”. As with most alpha releases, there are a few issues we’ve fixed and they’ll be in the next alpha…

Standard Autofocus Software

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JohemianKnapsody 23 July 2025 10:56 11

The first alpha is here - An alpha release of the v3 server

We’re hoping to have an improved version out soon, and feedback is always appreciated!

Topic		Replies	Views
Auto Focus On OFM General	5	240	20 August 2024
Need help adapting OFM software to a cheap microscope with motorized z axis Request Help	6	86	27 November 2025
Microscope server 2.6.0 beta 2 Announcements	0	415	16 October 2020
Automated Slide Scanning and Tiling General	48	2102	4 June 2025
Software Beta release Announcements	37	2548	22 November 2021

Standard Autofocus Software

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