Thanks Joel@ jc2450.
By the way I am using a high resolution optic module.
The above queries hold good even for “capture” option. .
What is the role of Z steps while defining step size and also number of steps during stack and scan?.
Everytime it tries to autofocus during stack and scan ,it keeps deviating from the mean value,and after some steps, the pictures totally lose clarity.
I have tried reducing the Z steps,but the results are same.
For example if I have 3 X steps,3 Y steps,and 5 Z steps…will the no of pics captured be 3x3x5=45 images?or
If I need to stack and scan the whole slide ,how many steps would it translate to ,considering the default step size of 2050 and 1500(approx).(tissue section approx 1cm x 1cm)
What direction will the slide be scanned,so as to correctly position the xy coordinates of the first image to be captured?
Please forgive my technical knowledge.