My image in openflexure which I just built from iorodeo like a black square with a 1 inch white circle in it. I used an objective lens that is 10x it’s an amscope 10 .25 160 .17. I was expecting to see just the image and not mostly black. Here is the image I see. Did I put something in backwards? Can anybody tell me what could be wrong?
Hi @techdog, that image does look unusual. You are using a low magnification lens, which can make setting up the illumination more difficult. Your screenshot is from the camera stage mapping step, have you already done the camera lens shading calibration? If you have, what did it look like before that calibration? Did you have a slide in place when you took this image? Does anything change if you move the slide?
If you click next on that screen, without doing the camera stage mapping, you will be in the main software. In settings under camera you can delete the lens shading table, which removes any artefacts that may have come from the camera lens shading calibration. What do you see then (with no slide on the stage)?
A picture of your microscope from the side might also help.
Finally did you get the plastic lens for the condenser from iorodeo as well? and the 50mm focal length tube lens for the optics module?
Hi. I found the problem, the raspberry pi camera lens was still on and had to be removed. Now the black box is gone, but it won’t autofocus. If I lift the slide up a little like 2-3 mm then it pops into focus. I’m not sure I understand why it’s not focusing and off by 2-3mm. Any ideas why something would be off by 2-3 mm? When I do autofocus is it adjusting Z slightly – it seems the range it checks is not very much. If I unscrew the lens camera assembly and shift it manually up and down I can get it to focus. Also my goals is to look at t-25 flask the bottom is about the thickness of two slides, any ideas on how to increase the autofocus range – the lens and camera assembly is currently up at the top of the keyhole, to focus I have to move it down if that’s any help.
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I just figured it out. I have to manually move Z first with the motors till it is on the plane I want, then autofocus. So everything is working. Yay.
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