Hi, I learned about your Openflexure project from the folks at IO rodeo .https://iorodeo.com/products/openflexure-microscope-kit
They provide a kit with various options. I opted for the high resolution version. I was thrilled to put microscope together. This is an exceptional instrument in my opinion Thank you!!!
I think the build went very well. I’m grateful for your excellent built instructions.
Some things I learned that may be of help to others.
My hdmi and usb cables did not insert well into the electronics drawer causing intermittent issues with the RPi booting and/or displaying. I filed out these areas to add clearance and had no further issues. It also may be worthwhile to verify your camera and light source are working before you install them. These can be verified before installing the electronics or the optics into the microscope.
There are various versions of the sanga board. Pre-read and follow instructions for the one you are using carefully. I think the build instructions i followed had images of an older version, and I got confused on where to plug in the light source, and learned the firmware is installed on the v0.5.4 board. There are directions on the forum for reloading firmware without a compiler.
It may be of value to wait on installation of the gear motors after you have successfully put a slide on the microscope and manually focused and adjusted. You may want to manually set the x, y, and z screws somewhere near mid range as well. Kind of establishes a “zero” point before going through the calibration section.