Sourcing non-printed parts

Ok thanks. I finally found it. Too bad the shipping is just as expensive as the kit…

oh? I guess that will vary depending on where you are - I didn’t think it was crazy from the UK, but I’ve not checked from elsewhere…

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See answer about camera compatibility below

Currently only the Pi Camera V2.1 is compatible with the software of the microscope.

There are optics modules that are designed to fit M12 cameras and the Logitech C270, but the camera feed can only be used through other general camera software, not integrated in the microscope control. These are listed towards the bottom of eth customisations page https://build.openflexure.org/openflexure-microscope/v7.0.0-beta1/customisation.html

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Thank you. So for my first build i want to keep things simple and use the stock cam. Even the resolution is rel. low compared to newer models. Should i obtain the regular Pi Camera V2.1 with IR filter or the one without IR filter (NoIR)?

Then, can someone recommend a lense for a High-resolution build aimed at doing blood, dark field imaging?

It’s worth noting that, while the newer cameras have more pixels than v2.1, the optical resolution (i.e. the smallest detail you can see) is generally limited by the microscope objective: adding more pixels to the camera will give you larger images, but won’t necessarily enable you to see anything more.

The LEDs we use for illumination don’t have much IR, so you ought not to notice much difference between the regular and NoIR versons, but I usually get the regular ones (the IR filter makes it a bit easier to clean if needed). For dark field blood imaging you probably want a 40x or 60x air objective - and I recommend the slightly more expensive “plan corrected” type. AmScope is a brand that strikes a decent compromise between quality and price in the £100 region. For our blood imaging we typically use a 100x oil immersion lens, but it’s harder to get dark field imaging working with this setup due to the very high numerical aperture of the objective.

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Thank you, this answers my questions. I will start with this 60 lense here and will probably upgrade to a 100 with oil immersion after i have some practice using the microscope.

Sourcing the non printed parts will still take some time. I will report my results as soon as the microscope is ready.

Super, let us know how you get on :slight_smile: As with the 40x vs 100x, the 60x lens will need your dark-field light to be coming in at a shallower angle in order to miss the objective - an NA of 0.85 means your illumination has to make an angle of less than 30 degrees to the microscope slide, whereas 0.65 means you can get away with 50 degrees, meaning you can use a smaller illumination ring, or place it further from the sample. Either should be possible though, depending on how you build it.

It’s also worth saying that the standard build is just for bright field - though there’s the LED array version that will do dark field, and also a forum thread from about a week ago doing dark field with a modified condenser.