I got the scope running well for the most part but the edges of the field of view are consistently blurry/a little out of focus compared to the center. Is there something physical with the scope I should be looking at that might help with this? Like the illumination being off center or the camera lens not sitting flat in the housing? Or is this an inherent limitation of using the basic quality camera module optics?
The opposite corners seem to be out of focus in the opposite direction (for example if I scroll from below the cells to above the cells the upper left will come into focus, then the center, then the lower right) which also makes me suspect the sample is just not level in the stage. But I’ve also tried leveling different sides of the stage by placing layers of tape under the sample to raise one side and that hasn’t seemed to change anything.
If anyone has ideas for other simple solutions I may have missed I’d love to hear them. Or if it would just be easier to image a grid with overlap and stitch the images together for an in focus composite?