I went ahead and made a close remodel of the OpenFlexure Delta Stage in Onshape since I don’t know OpenSCAD very well and cant really make custom modifications very easily. Along with this, I am working on modifications to use the new RPI High Quality with a 0.5 sensor I believe. I am trying to create a low-cost fluorescent microscope that can be used with an RNAScope or RNA tagging protocol. I am also going to do a few more modifications to allow for switching or rotating filter cubes automatically to allow for using multiple fluorophores for tagging the same RNA molecule to provide single RNA sensitivity.
I had a few questions on the optics design of the original OpenFlexure Microscope design. Is the cemented achromatic doublet lens meant to compensate for chromatic aberrations or is it just a tube length correction as listed in the build tutorial. If so do you think I could have issues with chromatic and spherical aberrations and do you have any recommendations for how to address these? I was also wondering if you had a diagram or dimensions for the distance from the objective and camera that the doublet lens was placed. Just trying to figure out how or if I need to modify these distances to work with the larger sensor or different tube lengths.
Thank you so much guys!