Stacking and scanning what software

That is a really nice image. The focus is sharp across the field of view which should give nice stacking and scanning. @JohemianKnapsody is developing a specific stack, scan and tile suite for the microscope, Automated Slide Scanning and Tiling - General - OpenFlexure Forum. I don’t know how far that is from being packaged in a way that can be used easily.

To get good registration for tiling the images in a scan you need good overlap, I think 30% of the frame overlapping with the previous is common. Once you have the images Fiji is one of the commonly used stitching algorithms, @dgrosen has used it quite a bit I think: Sharing an (almost) whole slide scan - Contributions - OpenFlexure Forum

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