Excessive color saturation in saved full-resolution images

Hello!
I brought the default and full size pictures to the same size.



Is it possible to somehow get rid of a full-sized image from neon shades? Thanks!

Do you mean the coloured fringes at the edges of features in the sample?
image
I suspect this is some combination of refraction of the light by the sample, and colour artefacts from the demosaicing process (where the Bayer-patterned images are turned into colour images). I’d be curous how much of it is “real” (i.e. you’d see it if you used eyepieces) and how much is a debayering artefact.

Given that your sample is probably transparent and more or less colourless (nice diatoms, by the way - you have a really cool variety of shapes), you could eliminate colour fringing entirely by using a monochrome image - probably the most artefact-free way to do this would be to extract just the green channel from the raw Bayer data. We’ve published a script that will extract the raw data into a TIF file, and with a bit more effort you can add back in the background-correction included in the JPEG images.

The two columns of images look the same to me?

WilliamW

maybe these examples will be clearer to you))



Notice the color bar at the bottom of the image
I think that this is the result of a different algorithm for low and full image resolution.

It is clear in those ones yes, The low resolution image at the bottom has a nice colour balance. The high resolution one above has green highlights throughout and a purple bar across the bottom. It almost looks as though the green image is mis-aligned. As Richard says, it could be a result of demosaicing process. If it is a real optical thing I would expect to see green/magenta fringes with opposite sense on opposite sides of the image. They would also change with focus. That happens a lot with high contrast scenes for normal cameras, but this looks different.

Richard, thanks for the answer, unfortunately, I can’t take the advice yet, I just looked quickly at what is written on the link. Yesterday, friends presented the Zeiss planachromat 40x but which was infinitely corrected with an M25 thread. I spent a lot of time reworking the model for my sizes and thread (I use openscad for the first time).
I will definitely try your advice, but first I need to finish the backlight (there is no condenser lens, now I light it for contrast through the hole) and try a different white temperature LED, the spectrum may not combine with the Bayer filters on the camera.
but it will all be later - today the motors arrived))

When you have a moment, it would be great if you could post a full resolution JPEG with bayer data (there’s a tick box in the software for that). The file size should be ~14Mb. It would be interesting to look at the raw Bayer data in case it tells us anything interesting.

If you’re using an infinity-corrected lens, we have recently discovered that it needs a taller microscope stand - there’s a revised STL on the forum in another thread already (apologies if you already found this, I can’t remember who was on that thread). I will try to get this added in to the instructions soon.

Forum did not accept the largest file…
archive 3 images in different resolutions default, resize and full with raw

Thanks for the link to .stl, but have not decided yet whether I will leave this lens in the design or not, in my opinion the magnification with it is much lower than with 40x 160

1 Like