Hi everyone! First post here. I’m building the high resolution microscope v7. Ok, here’s the thing: I bought a bunch of lenses for the illumination (here are the lenses I have: https://it.aliexpress.com/item/1005007275456146.html ). They fit perfectly and rest in the arm, no issues. But I don’t have …

Hi @giorgioFS . There is some information about the purpose of the illumination in the Knowledge Base section of the assembly instructions. To get a good image you need to have light coming onto your sample over the whole field of view at all angles. A piece of paper on the lens will do that. It is n…

Hi @WilliamW , thanks for the quick and detailed answer. I read about the PTFE and everything, but I didn’t fully understand (until now) why the lens exit point it’s not an ideal place. I’m a close-to-electronics engineer, so I decided on the LED workaround. But as mentioned before, I’m not too m…

A single colour of light can be helpful in some specific cases where there is information in a particular colour, but not usually. With a colour camera you already have red, green, blue colour channels available. The colourchanging LED is also just rgb. There is no actual yellow light, for example, …

Awesome! Thank you! Now enjoy your weekend, and sorry again for being so silly. Lots of information on where to start googling! Have a great day! ^^ And Thankyou SO SO SO MUCH!

Just to add to @WilliamW ’s comment: the NeoPixel RGB LEDs don’t have all three colours in the same place. This likely means the colour of the illumination isn’t constant across the field of view, which isn’t ideal. The diffuser is probably best placed next to the LED rather than next to the lens, b…

Does using a photography diffuser cut down to size make any sense? That’s what I used for my HQ build, but don’t know enough about microscopy to be able to tell if it is alright or introducing artifacts. If it works it might be an interesting and cheap alternative?

Also beware that the basic NeoPixels can have a fairly low PWM frequency (400Hz), so depending on your imaging setting you might encounter some flicker.

[image] WilliamW: We have recently found that the recommended polypropylene sheet for the diffuser is damaged by the light over long use. PTFE sheet looks as though it might be a better option, but we have not tried it yet. My offer to send anyone in the UK a PTFE diffuser for free to text/ex…

My memory is a sieve, I forgot that you had already suggested PTFE!

A small note regarding color. Depending on what’s being imaged, having a “monochromatic” LED, lens and camera can be helpful in some cases (e.g. imaging mechanical features). I use 450 nm LED, a flat field lens corrected for that one wavelength and a mono sony 462 chip – for imaging 1-micron partic…

Diffuse Led Light with paper: does it have meaning in this way?

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gerrit 12 December 2025 12:59 9

My offer to send anyone in the UK a PTFE diffuser for free to text/experiment with still stands:

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Diffuse Led Light with paper: does it have meaning in this way?

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